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Situated in Via della Lungara 233, the Guarini campus hosts the Aula Magna Regina auditorium as well as the Frohring Library, one of Italy's most comprehensive English language libraries. The Guarini campus also hosts numerous study rooms, media production facilities, a meditation garden, as well as JCU administrative offices.
JCU's Tiber Campus is located in Lungotevere Raffaello Sanzio 12, on the banks of Rome's famous Tiber river and a five-minute walk from Guarini. In addition to classrooms, the Campus houses offices such as Student Services and Immigration Services. It is also home to John Cabot University's cafeteria, the Tiber Cafe, which serves breakfast, lunch, and dinner and offers various meal plan options.
The Caroline Critelli Guarini Campus, situated in Piazza Giuseppe Gioachino Belli 11, is the first property owned by John Cabot University and it hosts classrooms, administrative offices, and a classroom that can be used for physics and basic electrical engineering courses. It was named for Caroline Critelli Guarini (1900 – 2001), mother the Hon. Frank J. Guarini, former Chairman of the JCU Board of Trustees and generous benefactor of the University.